Semi-nested polymerase chain reaction used for the rapid detection of bacteria and the Gram stain typing and its preliminary applications 快速检测细菌并革兰分型的半巢式聚合酶链反应及其初步应用
Application of Gram-stain in the diagnosis of bacterial vaginosis 革兰染色法在诊断细菌性阴道病中的应用
[ Method] All cases from the initial application of CMOS microscope were investigated retrospectively, 30 positive cases were selected respectively to compare with Clearview rapid diagnostic kit, isolating culture and Gram stain smear. 方法回顾性调查该科性病门诊自应用万倍显微镜以来的所有病例,分别选取30例患者与Clearview快速诊断试剂盒检测、分离培养及染色涂片检测结果进行比较。
Bacteria in colony were identified with gram stain and PCR method. 细菌通过革兰染色和PCR法鉴定。
Methods 318 samples of vaginal secret were observed by wet mount, Gram stain and were cultured. Cervical discharge and stool were cultured. 方法对318例女性患者阴道分泌物进行了湿片法检查和革兰染色显微镜检查,并对阴道分泌物、宫颈分泌物及粪便分别进行了假丝酵母菌培养。
The isolations were identified by gram stain, biochemical reactions and pathogenicity tests in mice. 从10个规模化猪场以直肠棉拭子采集PWD病料,经细菌分离纯化、革兰氏染色、生化试验和小鼠致病性试验等,对病原进行鉴定。
This study re-evaluated the application of Gram's stain in diagnosing gonorrhea in women by comparing with gonococci culture. 本文用淋球菌培养比较研究对革兰氏染色法在女性淋病诊断中的应用作重新评价。
Conclusion The results suggest that the elevated concentration of endotoxin in BALF> 1: 16 is superior to Gram stain for the rapid identification of patients with gram-negative pneumonia, and this test helps early administration of antimicrobial treatment. 结论结果提示,BALF中内毒素浓度≥1:16快速诊断阴性菌肺炎的准确性明显优于革兰染色检查,该方法有助于早期抗菌药物治疗的选择。
Each case was done by PAS, Giemsa, Gram, Grocott methenamine stain ( GMS), fast anti-acid stain and Warthin-starry stain. 每例均作PAS反应、吉姆萨染色、Gram染色、六胺银及抗酸染色和Warthinstarry染色。
This paper discusses the Gram stain principle in terms of the specific molecular structure and the different ionization state in different PH solutions of the triphenylmethane dye, which will ionize an ion with a positive electric when in the solution with PH higher than 3.2; 从染色剂三苯甲烷特有的分子结构、三苯甲烷染色剂在不同pH溶液中有不同的电离状态,当pH>3.2溶液时它将电离为带一个正电荷的离子的特质;
The coincidence rate between CMOS microscope and Clearview rapid diagnostic kit, isolating culture, Gram stain smear was all more than 90%. 万倍显微镜与Clearview快速诊断试剂盒检测、分离培养及染色涂片检测的检出符合率达90%以上。
Analgsis of Three-step Method and Four-step Method of Gram Stain 浅析革兰氏染色三步法、四步法
A comparative study of gram, s stain and gonococci culture in diagnosing gonorrhea in women 革兰氏染色与淋球菌培养诊断女性淋病的比较研究
The main pathogens ( 91.04%) of VAP was gram's stain negative germina. 其主要致病菌为革兰氏阴性菌,占91.04%。
Two strains of lactic acid bacteria ( SLlb, SLst) were screened through Gram stain, curd time observation and acid production analysis. 通过革兰氏染色、凝乳时间观察和产酸量分析,筛选出生长活力高,产酸能力强的2株酸乳生产菌株:SLlb和SLst。
Direct immunofluorescence staining technique using Gardnerella monoclonal antibody and Gram stain were used to examine the smears of abnormal vaginal secretion of 247 outpatients in Gynecology Department. Gardnerella vaginitis ( GV) was found in 6 cases ( 2.4%). 应用单克隆抗体直接免疫荧光染色,在妇科门诊247例异常阴道分泌物涂片中检出加德纳菌感染者6例(2.4%),诊断为加德纳杆菌性阴道炎。
Scrape the colonies after purification, and prepare to get the bacterial suspension. 2. Identification of Campylobacter jejuni: the gram stain, peroxidase test, hippuric acid hydrolysis test. 3. 鉴定空肠弯曲菌:革兰染色、过氧化物试验、马尿酸水解试验。